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Related Experiment Videos

Microsatellites can be misleading: an empirical and simulation study.

F Balloux1, H Brünner, N Lugon-Moulin

  • 1Institut d'Ecologie, Université de Lausanne, Switzerland. francois.balloux@esh.unibe.ch

Evolution; International Journal of Organic Evolution
|September 27, 2000
PubMed
Summary

Highly polymorphic microsatellite markers can underestimate genetic divergence. In common shrews (Sorex araneus), autosomal microsatellites showed lower differentiation than other markers due to high mutation rates.

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Area of Science:

  • Evolutionary genetics
  • Population genetics
  • Molecular ecology

Background:

  • Highly polymorphic genetic markers, such as microsatellites, can lead to underestimation of population divergence.
  • Microsatellite loci are prone to underestimation due to their high mutation rates.

Purpose of the Study:

  • To estimate genetic differentiation in a contact zone between two chromosome races of the common shrew (Sorex araneus).
  • To compare genetic differentiation estimates using autosomal microsatellites, a Y-chromosome microsatellite, and mitochondrial DNA.
  • To investigate the role of mutation rates and mutation models in microsatellite-based genetic differentiation.

Main Methods:

  • Analysis of 10 autosomal microsatellites, a novel Y-chromosome microsatellite, and mitochondrial DNA in Sorex araneus populations.

Related Experiment Videos

  • Comparison of genetic differentiation estimates using F- and R-statistics.
  • Simulations to assess the impact of microsatellite mutation rates and models on genetic differentiation estimates.

    • Main Results:

    • Autosomal microsatellites yielded significantly lower genetic differentiation estimates compared to Y-chromosome microsatellites, mitochondrial DNA, proteins, and karyotypes.
    • Simulations confirmed that high mutation rates (for F-statistics) and deviations from the single-step mutation model (for R-statistics) explain the discrepancy.
    • Sex-linked markers revealed that all gene flow between the two shrew races is female-mediated.

    Conclusions:

    • Autosomal microsatellites can underestimate genetic divergence in Sorex araneus due to their high mutation rates and model violations.
    • Female-mediated gene flow is dominant, suggesting potential male hybrid sterility as a barrier to male-mediated gene flow between chromosome races.