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Related Experiment Videos

Microbial sulfate reduction in a liquid-solid fluidized bed reactor.

S Nagpal1, S Chuichulcherm, L Peeva

  • 1Department of Chemical Engineering and Chemical Technology, Imperial College, London, SW7 2BY, UK.

Biotechnology and Bioengineering
|September 27, 2000
PubMed
Summary

This study demonstrates stable fluidized bed reactor operation for sulfate reduction using immobilized sulfate-reducing bacteria. Optimized reactor design can significantly enhance sulfate removal capacity.

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Area of Science:

  • Environmental Microbiology
  • Biochemical Engineering
  • Chemical Reaction Engineering

Background:

  • Sulfate reduction is crucial for wastewater treatment and resource recovery.
  • Immobilized microbial systems offer advantages in reactor stability and efficiency.
  • Fluidized bed reactors provide excellent mass transfer characteristics.

Purpose of the Study:

  • To evaluate the performance of a liquid-solid fluidized bed reactor for sulfate reduction using immobilized sulfate-reducing bacteria.
  • To investigate the effects of hydraulic retention time and biomass loading on reactor efficiency.
  • To develop and validate a mathematical model for reactor design and scale-up.

Main Methods:

  • Utilized a mixed culture of sulfate-reducing bacteria immobilized on porous glass beads in a fluidized bed reactor.

Related Experiment Videos

  • Controlled hydrogen sulfide (H2S) levels via nitrogen (N2) sparging.
  • Employed ethanol as the electron donor and carbon source.
  • Examined the impact of hydraulic retention time (HRT) and bead biomass loading on sulfate reduction rates and efficiency.

    • Main Results:

    • Achieved high sulfate reduction rates of up to 6.33 g sulfate L(-1) day(-1) at a 5.1 h HRT.
    • Demonstrated that sulfate reduction efficiency increases with HRT, becoming substrate-limited at 55h.
    • Developed a validated model predicting reactor performance and scale-up potential.

    Conclusions:

    • Stable and efficient sulfate reduction is achievable in a fluidized bed reactor with immobilized bacteria.
    • Reactor design, particularly the bed volume to liquid volume ratio, can significantly increase sulfate reduction capacity.
    • The developed model aids in the design and scale-up of similar bioreactors for effective sulfate removal.