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Related Experiment Videos

All solid-state GFP sensor.

Y Kostov1, C R Albano, G Rao

  • 1Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201-1503, USA.

Biotechnology and Bioengineering
|September 27, 2000
PubMed
Summary
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A novel all-solid-state green fluorescent protein (GFP) sensor was developed for accurate GFP measurement. This cost-effective sensor is robust against ambient light and scattering, enabling reliable online monitoring in fermentation.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Sensor Technology

Background:

  • Green fluorescent protein (GFP) is a vital reporter in biological research.
  • Accurate and real-time measurement of GFP is crucial for process monitoring.
  • Existing methods for GFP detection can be limited by interference and cost.

Purpose of the Study:

  • To develop and validate an all-solid-state sensor for quantifying green fluorescent protein (GFP).
  • To create a cost-effective and robust sensor suitable for online fermentation monitoring.
  • To assess the sensor's performance against ambient light and scattered excitation light.

Main Methods:

  • Construction of an all-solid-state GFP sensor.
  • Testing sensor performance with standard flow-through cuvettes.

Related Experiment Videos

  • Validation of the sensor in both off-line and on-line fermentation processes.
  • Evaluation of sensor response using UV or blue light emitting diodes (LEDs).

    • Main Results:

    • The developed sensor demonstrates immunity to ambient light interference.
    • It is practically insensitive to scattered excitation light in microbial suspensions.
    • The sensor exhibits a wide measurement range (0.0002-1 g/L) with a low limit of detection (0.00019 g/L).
    • Successful on-line monitoring of fermentation processes was achieved.

    Conclusions:

    • The all-solid-state GFP sensor offers a reliable, cost-effective solution for GFP quantification.
    • Its robustness and ease of use make it suitable for various applications, including fermentation monitoring.
    • The sensor provides a valuable tool for real-time biological process analysis.