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Related Experiment Videos

Laboratory experience with a radiometric method for detecting bacteremia.

W A Thiemke, K Wicher

    Journal of Clinical Microbiology
    |March 1, 1975
    PubMed
    Summary
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    The automated radiometric BACTEC system detected positive blood cultures faster than the conventional method. While BACTEC showed quicker results for aerobic and anaerobic bacteria, the conventional method identified slightly more cultures overall.

    Area of Science:

    • Medical Microbiology
    • Clinical Diagnostics
    • Bacteriology

    Background:

    • Blood culture is crucial for diagnosing bloodstream infections.
    • Traditional methods require time for microbial growth and detection.
    • Automated systems aim to improve speed and sensitivity in blood culture analysis.

    Purpose of the Study:

    • To compare the diagnostic performance of the automated radiometric BACTEC system against a conventional laboratory method for detecting bacteria in blood.
    • To evaluate the time to positivity and detection rates for aerobic and anaerobic organisms, as well as fungi.

    Main Methods:

    • A comparative study involving 3,045 blood specimens from 804 patients.
    • Inoculation of blood into BACTEC bottles (30 ml total) and conventional media bottles (50 ml total).

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  • Incubation and monitoring of cultures for microbial growth using both systems.

    • Main Results:

    • The BACTEC system detected positive blood cultures as early as 6 hours.
    • Within 24 hours, BACTEC identified 77% of aerobic organisms versus 48% for the conventional method.
    • BACTEC detected 100% of anaerobic cultures within 4 days (vs. 74% conventional) and 67% of fungi by day 4 (vs. 27% conventional).
    • The conventional system detected 5 more positive cultures overall.
    • 208 false positive results were recorded with BACTEC.

    Conclusions:

    • The BACTEC system offers significantly faster detection of positive blood cultures, particularly for aerobic bacteria and fungi.
    • While BACTEC excels in speed, the conventional method showed a slightly higher overall detection rate in this study.
    • The BACTEC system demonstrates potential for earlier diagnosis of bacteremia, though false positive results require consideration.