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Updated: Aug 15, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Simulation of electrophoretic separations by the flux-corrected transport method
1Department of Chemical and Environmental Engineering, The University of Arizona, Tucson 85721. USA.
Abstract:
Electrophoretic separations at typical experimental electric field strengths have been simulated by applying the flux-corrected transport (FCT) finite difference method to the transient, one-dimensional electrophoresis model. The performance of FCT on simulations of zone electrophoresis (ZE), isotachophoresis (ITP), and isoelectric focusing (IEF) has been evaluated. An FCT algorithm, with a three-point, central spatial discretization, yields numerical solutions without numerical oscillations or spurious peaks, which have plagued previously-published second-order solutions to benchmark ZE and ITP problems. Moreover, the FCT technique captures sharp zone boundaries and IEF peaks more accurately than previously-published, first-order upwind schemes.
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