Related Experiment Videos
A novel immunoadsorbent for rheumatoid arthritis therapy--preparation and efficacy evaluation
1Institute for Molecular Biology, Bioactive Material Research Laboratory, Nankai University, PR China.
Aim:
To develop a novel immunoadsorbent for rheumatoid arthritis (RA) therapy.
Methods:
A RA immunoadsorbent was developed by binding heat-aggregated human IgG(HAHIgG) to porous agar gel beads. Its adsorption capacity for rheumatoid factors (RFs), storage stability and blood compatibility were evaluated.
Results:
The coupling yield of HAHIgG on the carrier was 6.0 mg/g wet gel. Saturation adsorption capacity of the adsorbent for IgMRF, IgGRF and IgARF were 3400, 2240 and 2400 IU/g, respectively. The adsorbent can be stored at 4 degrees C for three months without significant variance in its activity. Its fine permeability and hemocompatibility were demonstrated by extracorporeal hemoperfusion on rabbits.
Conclusion:
HAHIgG/agar gel is a safe and effective immunoadsorbent for RA therapy, its potential clinical use is promising in the future.