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A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus
X Chen1, M Stone, C Schlagnhaufer
1Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Applied and Environmental Microbiology
|September 30, 2000
Abstract:
We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.