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A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes

A E Bernhard1, K G Field

  • 1Department of Microbiology, Oregon State University, Corvallis, Oregon 97330, USA.

Applied and Environmental Microbiology
|September 30, 2000
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...