Antisense silencing of the creA gene in Aspergillus nidulans
L F Bautista1, A Aleksenko, M Hentzer
1Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, 2800 Lyngby, Denmark.
Applied and Environmental Microbiology
|September 30, 2000
Abstract:
Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.
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