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Atomic co-ordinates for yeast phenylalanine tRNA.
Nucleic Acids Research
|September 1, 1975
Summary
Atomic coordinates for yeast phenylalanine transfer RNA (tRNA-Phe) were determined at 2.5 A resolution. This structural data was derived from a wire model fitted to an electron density map using isomorphous replacement methods.
Area of Science:
- Biochemistry
- Structural Biology
- Molecular Biology
Background:
- Transfer RNA (tRNA) molecules are essential for protein synthesis, mediating the translation of genetic code into amino acid sequences.
- Understanding tRNA structure is crucial for elucidating its function in translation and its interactions with ribosomes and aminoacyl-tRNA synthetases.
Purpose of the Study:
- To present the atomic coordinates of yeast tRNA(Phe).
- To provide a high-resolution structural model of yeast tRNA(Phe) for further functional and mechanistic studies.
Main Methods:
- X-ray crystallography was employed to determine the structure.
- Isomorphous replacement techniques were used to obtain the electron density map.
- A wire skeletal model was fitted to the 2.5 A resolution electron density map to derive atomic coordinates.
Main Results:
- The study presents detailed atomic coordinates for yeast tRNA(Phe).
- The structure was resolved at a resolution of 2.5 Angstroms, providing a detailed view of the molecule.
- The derivation involved fitting a wire skeletal model to an electron density map obtained via isomorphous replacement.
Conclusions:
- The determined atomic coordinates provide a foundational structural dataset for yeast tRNA(Phe).
- This high-resolution structure facilitates detailed analysis of tRNA-Phe's three-dimensional conformation and its implications for biological function.