Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antifungal susceptibility testing: progress and future developments.

M A Pfaller1

  • 1Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA. michael-pfaller@uiowa.edu

The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases
|September 30, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wild-type MIC distributions and epidemiological cutoff values for the echinocandins and Candida spp.

Journal of clinical microbiology·2009
Same author

Activity of MGCD290, a Hos2 histone deacetylase inhibitor, in combination with azole antifungals against opportunistic fungal pathogens.

Journal of clinical microbiology·2009
Same author

In vitro susceptibility of clinical isolates of Aspergillus spp. to anidulafungin, caspofungin, and micafungin: a head-to-head comparison using the CLSI M38-A2 broth microdilution method.

Journal of clinical microbiology·2009
Same author

In vitro activity of seven systemically active antifungal agents against a large global collection of rare Candida species as determined by CLSI broth microdilution methods.

Journal of clinical microbiology·2009
Same author

Wild-type MIC distribution and epidemiological cutoff values for Aspergillus fumigatus and three triazoles as determined by the Clinical and Laboratory Standards Institute broth microdilution methods.

Journal of clinical microbiology·2009
Same author

Global view of antimicrobial resistance. Findings of the SENTRY Antimicrobial Surveillance Program, 1997-1999.

Postgraduate medicine·2009
Same journal

Risk factors associated with invasive fungal disease post-liver transplantation.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same journal

Microbiome analysis of Cystic Fibrosis sputum presents higher sensitivity than the conventional bacterial culture.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same journal

Low awareness of HIV status and high proportion of recent infections among young travestis and trans women in Brazil.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same journal

Rare clinical presentations of paracoccidioidomycosis: Epidemiological, clinical, and microbiological insights from a Brazilian reference center.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same journal

Immunization against respiratory viruses among dental surgeons: high personal coverage, low involvement in vaccine promotion.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same journal

Is candidemia mortality inevitable or a consequence of inadequate guideline adherence?

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
See all related articles

Standardized antifungal susceptibility testing methods for yeasts and molds have advanced significantly. These methods improve clinical utility, aid in developing commercial tests, and guide future research in antifungal resistance.

Area of Science:

  • Clinical Microbiology
  • Mycology
  • Pharmacology

Background:

  • Standardized broth reference methods for antifungal susceptibility testing (AST) of yeasts are now established.
  • Adaptations for yeast and mold testing have increased clinical utility and laboratory capabilities.
  • Quality control limits and minimum inhibitory concentration (MIC) breakpoints are available for key antifungal agents.

Purpose of the Study:

  • To review recent developments in antifungal susceptibility testing methods.
  • To highlight the clinical utility and standardization of yeast and mold AST.
  • To discuss the role of reference methods in developing commercial AST products and future research.

Main Methods:

  • Adaptation of the reference macrodilution method to a microdilution format (M27-A).

Related Experiment Videos

  • Development and validation of quality control limits and MIC breakpoints.
  • Evaluation of commercial AST products (Sensititre, KPI, Etest) as alternatives to reference methods.

    • Main Results:

    • The M27 microdilution method is feasible for both yeast and mold susceptibility testing.
    • Commercial methods show promise as viable alternatives for yeast AST, with Etest also applicable to molds.
    • Established reference methods provide a benchmark for developing user-friendly and standardized commercial tests.

    Conclusions:

    • Antifungal susceptibility testing methods have significantly advanced, enhancing clinical decision-making.
    • Standardized methods and commercial products are crucial for monitoring antifungal resistance trends.
    • Future research will focus on detecting resistance, expanding mold testing guidelines, and clinical applications.