Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genotyping of hepatitis C virus isolates using CLIP sequencing.

R S Ross1, S O Viazov, C D Holtzer

  • 1Institute of Virology, National Reference Centre for Hepatitis C, University of Essen, D-45122 Essen, Germany. stefan.ross@uni-essen.de

Journal of Clinical Microbiology
|October 4, 2000
PubMed
Summary

The TRUGENE HCV 5'NC genotyping kit shows reliable performance for hepatitis C virus (HCV) detection. While it struggles with subtype differentiation for genotypes 1 and 2, it accurately identifies major HCV types for clinical use.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clearance of HBV DNA in immunized children born to HBsAg-positive mothers, years after being diagnosed with occult HBV infection.

Journal of viral hepatitis·2015
Same author

Control of hepatitis B virus infection in hematopoietic stem cell recipients after receiving grafts from vaccinated donors.

Bone marrow transplantation·2015
Same author

Performance characteristics of the VERSANT hepatitis C virus RNA 1.0 (kPCR) assay.

International journal of medical microbiology : IJMM·2015
Same author

Adhesive luting of new CAD/CAM materials.

International journal of computerized dentistry·2015
Same author

[Combined hepatitis A/B vaccination: evaluation of a vaccination schedule in facilities for handicapped people].

Gesundheitswesen (Bundesverband der Arzte des Offentlichen Gesundheitsdienstes (Germany))·2013
Same author

Pulse-triggered DTI sequence with reduced FOV and coronal acquisition at 3T for the assessment of the cervical spinal cord in patients with myelitis.

AJNR. American journal of neuroradiology·2012

Area of Science:

  • Virology
  • Molecular Diagnostics
  • Hepatology

Background:

  • Accurate hepatitis C virus (HCV) genotyping is crucial for patient management and prognosis.
  • New diagnostic tools are needed to improve the efficiency and reliability of HCV genotyping.

Purpose of the Study:

  • To evaluate the specificity and reliability of the TRUGENE HCV 5'NC genotyping kit.
  • To compare the TRUGENE kit's performance against established methods like DNA enzyme immunoassay (DEIA).

Main Methods:

  • Utilized PCR fragments from the Roche AMPLICOR HCV test for simultaneous PCR amplification and CLIP sequencing of the 5' noncoding region (5'NCR).
  • Genotyped 100 HCV isolates using both the TRUGENE HCV 5'NC kit and DEIA.
  • Performed phylogenetic analysis on discordant samples using HCV core and NS5B region primers.

Related Experiment Videos

Main Results:

  • Achieved complete concordance between TRUGENE and DEIA in 91% of cases.
  • TRUGENE kit showed limitations in differentiating subtypes of genotypes 1 and 2 due to 5'NCR conservation.
  • No misclassification occurred between major HCV genotypes (1 vs. non-1).

Conclusions:

  • The TRUGENE HCV 5'NC genotyping kit is a reliable tool for clinical HCV genotyping, particularly for identifying major genotypes.
  • The kit offers a fast and convenient option for laboratories already employing the Roche AMPLICOR HCV test.
  • Further validation may be needed for precise subtype differentiation within specific genotypes.