Related Experiment Video
Updated: Jul 14, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Published on: October 15, 2016
Structure, function, and control of phosphoinositide-specific phospholipase C
1Departments of Anesthesiology and Physiology and Biophysics, School of Medicine, State University of New York, Stony Brook, New York 11794, USA. Rebecchi@epo.sunysb.edu
Phosphoinositide-specific phospholipase C (PLC) enzymes are crucial for cell signaling. Recent research reveals specific roles for PLC subtypes in cellular responses and development across various organisms.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Phosphoinositide-specific phospholipase C (PLC) enzymes are key phosphodiesterases.
- They cleave inositol lipids, generating essential second messengers: inositol 1,4,5-trisphosphate and diacylglycerol.
- PLCs are activated by various cell surface receptors.
Purpose of the Study:
- To review advances in understanding PLC subtypes (beta, gamma, delta).
- To discuss their molecular regulation and biological functions across diverse organisms.
- To highlight specific roles in cell signaling and development.
Main Methods:
- Crystallographic studies of PLC-delta(1) for structural insights.
- Cellular studies to uncover PLC activity regulation.
- Genetic studies in yeast, slime molds, plants, flies, and mammals.
Main Results:
- New insights into PLC multidomain arrangement and catalytic mechanisms.
- Identification of novel modes for controlling PLC activity.
- Demonstration of coordinated action among PLC isoforms in cellular responses.
Conclusions:
- PLC isoforms have specific and conditional roles in cell signaling and development.
- Understanding PLC function is critical for deciphering complex cellular processes.
- Further research across species will elucidate precise biological functions.
More Related Videos
08:07Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Published on: July 26, 2019
10:31A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
Published on: September 26, 2025
Related Concept Videos
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
IP3/DAG Signaling Pathway