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Published on: August 23, 2019
In vitro digestibility of bacillus fermented soya bean
J L Kiers1, A E Van Laeken, F M Rombouts
1Wageningen University, Agrotechnology and Food Sciences, Laboratory of Food Microbiology, The Netherlands. jeroen.kiers@micro.fdsci.wag-ur.nl
Bacillus fermentation significantly enhances the nutritional value of soybeans by increasing soluble matter and improving in vitro digestibility. This process breaks down complex molecules, making nutrients more available for absorption.
Area of Science:
- Food Science
- Microbiology
- Biotechnology
Background:
- Bacillus fermentation is crucial for traditional legume products like natto and kinema.
- Bacillus subtilis is a key species in these fermentations.
Purpose of the Study:
- To investigate the impact of Bacillus fermentation on soybean macromolecules.
- To quantify changes in soluble matter, digestibility, and nutrient availability.
Main Methods:
- Fermentation of autoclaved soybeans with Bacillus species for 48 hours.
- Analysis of soluble and dialyzable matter.
- Assessment of protein and carbohydrate degradation.
- In vitro digestibility assays.
Main Results:
- Soluble matter increased from 22% to 65%; dialyzable matter rose from 6% to 40%.
- Significant protein and carbohydrate degradation occurred within the first 18 hours.
- In vitro digestibility increased from 29% to 33-43%.
Conclusions:
- Bacillus fermentation effectively degrades soybean macromolecules into water-soluble, low-molecular-weight compounds.
- This process substantially enhances nutrient availability, as evidenced by increased in vitro digestibility.
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