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Patch testing with neomycin sulfate.

B Bjarnason¹, E Flosadóttir

¹Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.

Contact Dermatitis

|October 4, 2000

Summary

Lower neomycin sulfate concentrations on polyester squares effectively identified positive patch tests. This method showed promise compared to standard patch testing, with potential for improved accuracy in diagnosing neomycin allergy.

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Area of Science:

Dermatology
Allergology
Contact Dermatitis

Background:

The standard patch test concentration for neomycin sulfate is 20% in petrolatum, applied occluded for 48 hours.
The efficiency of this standard test is often questioned due to potential variations in allergen dosage.

Purpose of the Study:

To compare the diagnostic efficiency of lower neomycin sulfate concentrations with the standard 20% concentration.
To evaluate different vehicles (petrolatum and demineralized water) and application methods for neomycin sulfate patch testing.

Main Methods:

16 neomycin-sulfate positive subjects were retested using serial doses (0.4 to 0.0085 mg/cm2) on polyester squares applied via tape and foil.
Tests were also conducted using 20% neomycin sulfate in petrolatum within Finn Chambers and applied directly with transparent foils.
Readings were taken between 3 and 264 hours post-application.

Main Results:

Foil application of polyester squares with 7% of the standard dose successfully distinguished between visually positive and negative subjects.
This method identified subjects with neomycin allergy more effectively than standard patch testing.
Two subjects showed false-negative results with the foil method but doubtful responses with Finn Chambers.

Conclusions:

Lower doses of neomycin sulfate applied on polyester squares via foil present a potentially more sensitive method for patch testing.
This approach may improve the diagnosis of neomycin hypersensitivity compared to traditional methods.
Further research is warranted to optimize this technique and minimize false-negative results.

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