Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Receptor editing in developing T cells.

M A McGargill1, J M Derbinski, K A Hogquist

  • 1Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.

Nature Immunology
|March 23, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation.

Immunity·2002
Same author

A low affinity TCR ligand restores positive selection of CD8+ T cells in vivo.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Signal strength in thymic selection and lineage commitment.

Current opinion in immunology·2001
Same author

T cell receptor editing.

Immunology letters·2001
Same author

Assays of thymic selection. Fetal thymus organ culture and in vitro thymocyte dulling assay.

Methods in molecular biology (Clifton, N.J.)·2000
Same author

Positive selection is limited by available peptide-dependent MHC conformations.

Journal of immunology (Baltimore, Md. : 1950)·2000
Same journal

Editorial Expression of Concern: Recognition of the nonclassical MHC class I molecule H2-M3 by the receptor Ly49A regulates the licensing and activation of NK cells.

Nature immunology·2026
Same journal

Inflammatory immune modulators of AML lung infiltration and respiratory failure.

Nature immunology·2026
Same journal

The neuroimmune system and cognition.

Nature immunology·2026
Same journal

Critical connections.

Nature immunology·2026
Same journal

Innate immune signaling and functions in astrocytes.

Nature immunology·2026
Same journal

Epigenetic mechanisms of inflammatory memory in the central nervous system.

Nature immunology·2026
See all related articles

Developing T cells normally die when encountering self-antigens. Instead, thymic antigen presentation induced T cell receptor editing, a mechanism preventing self-reactive T cells and maintaining self-tolerance.

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • T cell development is crucial for immune system function.
  • Negative selection via apoptosis eliminates T cells recognizing self-antigens, preventing autoimmunity.
  • The role of thymic epithelial cells in T cell selection requires further elucidation.

Purpose of the Study:

  • To investigate the consequences of self-antigen presentation by thymic epithelial cells on T cell development.
  • To explore alternative mechanisms to apoptosis in T cell tolerance induction.
  • To understand the implications of T cell receptor editing in the thymus.

Main Methods:

  • Generation of transgenic mice expressing a peptide antigen in thymic cortical epithelial cells.
  • Analysis of T cell populations and T cell receptor expression in the thymus.

Related Experiment Videos

  • Assessment of gene rearrangement at the endogenous T cell receptor alpha locus.

    • Main Results:

    • Antigen presentation by thymic epithelial cells did not induce T cell deletion (apoptosis).
    • Instead, T cell receptor internalization was observed upon antigen encounter.
    • Increased gene rearrangement at the TCR alpha locus, indicating receptor editing, was detected in developing T cells.

    Conclusions:

    • Thymic antigen presentation can trigger T cell receptor editing as a mechanism to avoid self-reactivity.
    • Receptor editing in T cells parallels mechanisms observed in B cell development.
    • This finding expands our understanding of T cell selection, self-tolerance, and immune regulation.