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Autofluorescence Imaging to Evaluate Cellular Metabolism
Published on: November 15, 2021
Imaging sustained dissipative patterns in the metabolism of individual living cells
H R Petty1, R G Worth, A L Kindzelskii
1Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA. hpetty@biology.biosci.wayne.edu
Physical Review Letters
|October 4, 2000
Abstract:
Theoretical studies have predicted spatiotemporal organization of cell metabolism. Using a rapidly gated CCD camera, we demonstrate for the first time sustained traveling waves of NAD(P)H autofluorescence and protons in individual morphologically polarized living cells. Chemical concentration fronts moved in the direction of cell orientation, thus correlating dissipative structures with cell shape.
