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Updated: Aug 14, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular
C Castagné1, H Terenzi, M M Zakin
1Laboratoire de Résonance Magnétique Nucléaire, CNRS URA 1773, Institut Pasteur, 28, rue du Dr.-Roux, 75724 cedex 15, Paris, France.
Abstract:
Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta, showed large shifts of imino protons and 31P signals, suggesting major conformational changes.
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