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Updated: Oct 11, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
Published on: June 26, 2020
Expression of DNA-dependent protein kinase catalytic subunit and Ku80 in developing human brains: implication of
1Division of Child Neurology, Institute of Neurological Sciences, Tottori University, Yonago, 683-8504, Tottori, Japan. oka@grape.med.totorri-u.ac.jp
Abstract:
We investigated expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and Ku80 in developing human brain, both of which have been suggested to be involved in the repair of DNA double-strand break (DSB). Their expressions were well correlated, and the highest immunoreactivity was observed in post-mitotic immature neurons in the cerebral cortex as well as in progenitors in the periventricular germinal layer. The reactivity gradually decreased during development. Our results support the notion that DSB is generated during the ontogeny of the human central nervous system.
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