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Characterization of Brassica S-haplotypes lacking S-locus glycoprotein
T Suzuki1, M Kusaba, M Matsushita
1Institute of Radiation Breeding, National Institute of Agrobiological Resources, P.O. Box 3, Ohmiya-machi, Naka-gun, Ibaraki 319-2293, Japan.
Abstract:
Self-incompatibility (SI) in Brassica is regulated by a single multi-allelic locus, S, which contains highly polymorphic stigma-expressed genes, SLG and SRK. While SRK is shown to be the determinant of female SI specificity, SLG is thought to assist the function of SRK. Here we report that the SLG genes of self-incompatible S(18) and S(60) homozygotes of Brassica oleracea have an in-frame stop codon and a 23 bp deletion resulting in a frame-shift, respectively. The finding that these SLG genes do not encode functional SLG proteins suggests that SLG is not essential for SI. The possible role of SLG in SI was discussed.