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Rapid serum vancomycin assay by high-performance liquid chromatography using a semipermeable surface packing material
I Furuta1, T Kitahashi, T Kuroda
1Department of Clinical Pathology, Kinki University School of Medicine, Osakasayama, Japan. itaru@med.kindai.ac.jp
Summary
A new high-performance liquid chromatography (HPLC) method offers a simple, rapid, and economical way to measure vancomycin in serum. This assay provides accurate quantification without interference from other drugs.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
Background:
- Vancomycin is a critical antibiotic for treating serious infections.
- Accurate and efficient monitoring of vancomycin serum levels is essential for therapeutic efficacy and minimizing toxicity.
Purpose of the Study:
- To develop and validate a novel isocratic high-performance liquid chromatographic (HPLC) assay for the direct quantification of vancomycin in human serum.
- To establish the assay's sensitivity, linearity, reproducibility, and accuracy, and assess its potential for clinical application.
Main Methods:
- Direct injection of serum microquantities onto an octyl-C(8) silica column.
- Isocratic elution using a mobile phase of disodium hydrogen phosphate buffer (pH 7.0) and acetonitrile.
- Detection of vancomycin at 240 nm.
Main Results:
- The assay demonstrated a minimum limit of detection of 0.5 microg/ml.
- Excellent linearity was observed from 0 to 100 microg/ml (r=0.995).
- High reproducibility (within-run CV 1.1-2.7%, day-to-day CV 3.1-4.0%) and recovery (94-105%) were achieved, with no interference from 41 other drugs.
Conclusions:
- The developed HPLC method provides a simple, rapid, and economical approach for serum vancomycin quantification.
- This assay is highly accurate, reproducible, and specific, making it suitable for routine clinical laboratory use.