Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Do androgens have a direct effect on endometrial function? An in vitro study.

E M Tuckerman1, M A Okon, T Li

  • 1Biomedical Research Unit, Jessop Hospital for Women, Sheffield, United Kingdom.

Fertility and Sterility
|October 6, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The relationship between serum prolactin concentration and pregnancy outcome in women with unexplained recurrent miscarriage.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·2013
Same author

Expression of leukaemia inhibitory factor and interleukin 15 in endometrium of women with recurrent implantation failure after IVF; correlation with the number of endometrial natural killer cells.

Human reproduction (Oxford, England)·2012
Same author

SUBCUTANEOUS FIBROID SYPHILOMATA.

The British journal of venereal diseases·2011
Same author

Gumma of Muscle.

The British journal of venereal diseases·2011
Same author

Anti-Syphilitic Treatment and Hepatic Cirrhosis.

The British journal of venereal diseases·2011
Same author

III. The Need for Further Powers to Deal with Sources of Infection, Contacts and Defaulters.

The British journal of venereal diseases·2011
Same journal

Minimally Invasive, Fertility-Sparing Surgical Management of Multiple Bilateral Ovarian Dermoid Cysts.

Fertility and sterility·2026
Same journal

Preconception dietary patterns in association with live birth and pregnancy loss: a couples-based approach.

Fertility and sterility·2026
Same journal

Corrigendum to "Clinical management of mosaic results from preimplantation genetic testing for aneuploidy of blastocysts: a committee opinion" [Fertil Steril 2023;120:973-82].

Fertility and sterility·2026
Same journal

Expression of concern 'Adhesion formation after intracapsular myomectomy with or without adhesion barrier' [Fertil Steril 2011; 95:1780-5].

Fertility and sterility·2026
Same journal

Ovarian stimulation with follitropin δ is safe and effective: results from the RITA randomized, double-blind, placebo-controlled trials.

Fertility and sterility·2026
Same journal

Reply of the authors: 'Transvaginal ultrasound as a guiding tool in the management for deep endometriosis'.

Fertility and sterility·2026
See all related articles

Androstenedione, but not other androgens, was found to inhibit human endometrial cell growth and function in vitro. This suggests high androgen levels may adversely affect the endometrium, potentially contributing to infertility and miscarriage.

Area of Science:

  • Reproductive Endocrinology
  • Cell Biology
  • Gynecology

Background:

  • Androgens are implicated in reproductive health.
  • Their direct impact on endometrial epithelial cells requires elucidation.
  • Understanding these effects is crucial for reproductive medicine.

Purpose of the Study:

  • To investigate the direct effects of androgens on human endometrial epithelial cell function.
  • To determine if androstenedione, testosterone, dihydrotestosterone, or DHEA influence cell growth and secretion.

Main Methods:

  • In vitro study using human endometrial epithelial cells from fertile women.
  • Cells were incubated with various androgens (androstenedione, testosterone, dihydrotestosterone, DHEA).
  • Assessed glycodelin A secretion, (3)H-thymidine uptake, and Ki67 expression; androgen receptor presence confirmed.

Related Experiment Videos

Main Results:

  • Androstenedione (10(-6) M and 10(-7) M) significantly decreased glycodelin A secretion and cell proliferation markers in a dose-dependent manner.
  • Testosterone, dihydrotestosterone, and DHEA showed no significant effects on these parameters.
  • Androgen receptor presence confirmed, and effects were blocked by cyproterone acetate, verifying direct action.

Conclusions:

  • Androstenedione directly inhibits human endometrial epithelial cell growth and secretory activity.
  • Elevated androgen levels, as seen in polycystic ovary syndrome, may negatively impact endometrial function.
  • This adverse effect could contribute to infertility and miscarriage.