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Interaction between inflammatory cells and heparin-surface-modified intraocular lens.
T Tanaka1, N Yamakawa, T Mizusawa
1Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. takaoeye@tkh.att.ne.jp
Journal of Cataract and Refractive Surgery
|October 6, 2000
Summary
Activated lymphocytes adhere to heparin-surface-modified intraocular lenses (HSM IOLs). Stimulants like interphotoreceptor retinoid-binding protein and concanavalin A (ConA) increase this inflammatory cell interaction on HSM IOL surfaces.
Area of Science:
- Ophthalmology
- Biomaterials Science
- Immunology
Background:
- Intraocular lenses (IOLs) are crucial for vision restoration after cataract surgery.
- Inflammatory cell adherence to IOL surfaces can lead to complications.
- Heparin surface modification (HSM) is explored to improve IOL biocompatibility.
Purpose of the Study:
- To investigate the interaction and adherence of inflammatory cells to heparin-surface-modified intraocular lenses (HSM IOLs).
Main Methods:
- Rat splenic mononuclear leukocytes from experimental autoimmune uveitis models were cultured with HSM IOL optics.
- Adherent cell counts were measured with and without interphotoreceptor retinoid-binding protein and concanavalin A (ConA).
- Adherent cells were analyzed using light and scanning electron microscopy.
Main Results:
- Interphotoreceptor retinoid-binding protein and ConA significantly increased inflammatory cell adherence to the HSM IOL surface compared to controls.
- The majority of adherent cells were identified as lymphocytes, appearing small and round.
Conclusions:
- Activated lymphocytes demonstrate a tendency to adhere to the surface of heparin-surface-modified intraocular lenses.
- HSM IOLs may influence inflammatory cell behavior, warranting further investigation for clinical implications.