Related Experiment Video
Updated: Jul 17, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants
R Anandalakshmi1, R Marathe, X Ge
1Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.
Abstract:
Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.
Related Concept Videos
Co-activators and Co-repressors
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
lncRNA - Long Non-coding RNAs
Co-activators and Co-repressors
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

