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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
08:30

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Published on: May 16, 2012

[Detection of PSA mRNA and PSMA mRNA by means of RT-PCR]

T Deguchi1, N Yamamoto, H Ehara

  • 1Department of Urology, Gifu University School of Medicine.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|October 7, 2000
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...