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H+-induced tension development in demineralized dentin matrix
D H Pashley1, Y Zhang, R M Carvalho
1Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta 30912-1129, USA. dpashley@mail.mcg.edu
Journal of Dental Research
|October 7, 2000
Summary
Acidic solutions cause demineralized dentin matrix to contract, potentially leading to matrix collapse. This effect is linked to hydrogen ion concentration and pre-strain, suggesting collagen conformation changes.
Area of Science:
- Biomaterials Science
- Dental Materials
- Biochemistry
Background:
- Atomic force microscopy studies indicate dentin surface recession after acid-etching.
- Understanding the mechanical behavior of demineralized dentin matrix is crucial for dental applications.
Purpose of the Study:
- To test the hypothesis that acidic solutions induce contraction in demineralized dentin matrix.
- To quantify the contractile stress developed in demineralized dentin under acidic conditions.
Main Methods:
- Human third molar dentin beams were demineralized using EDTA.
- Specimens were subjected to acidic solutions in a tester to record tension development.
- Variables included gauge length, cross-sectional area, pre-strain, and pH.
Main Results:
- Immersion in acidic solutions induced tension in demineralized dentin.
- Tension was directly proportional to H+ concentration (pH < 2) and pre-strain.
- Contractile stress ranged from 0.2-0.4 MPa, sufficient to cause matrix collapse.
Conclusions:
- Acidic solutions can cause significant contraction of demineralized dentin matrix.
- The observed contraction is likely mediated by H+-induced conformational changes in the collagen matrix.
- These findings have implications for understanding dentin behavior during dental procedures.