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Human TP53 from the malignant glioma-derived cell lines M059J and M059K has a cancer-associated mutation in exon 8
C W Anderson1, M J Allalunis-Turner
1Biology Department, Brookhaven National Laboratory, Upton, New York 11973-5000, USA.
Radiation Research
|October 7, 2000
Abstract:
The protein coding segment of the TP53 genes from the glioma-derived cell lines M059J and M059K was sequenced. The sequences from both cell lines were identical over 5039 bp, including the gene segment containing exons 2 through 9, exon 10, and the proximal segment of exon 11. In both cells, the first nucleotide of codon 286 (GAA, Glu) is changed to an A (AAA, Lys). Comparison with the same TP53 segment from the A549 human lung carcinoma cell line revealed several differences in intron sequence.