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HER2 promoter controlled specific expression of the reporter gene in ovarian cancer cell line

S Zheng¹, S Wang, L Ma

¹Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 P.R.China. wdn@soho.com

Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics

|October 12, 2000

Abstract

Objective:

Construct a retrovirus vector that expresses the downstream gene specifically in ovarian cancer cells so as to increase the specificity of gene therapy.

Methods:

HER2 promoter obtained by PCR was cloned into P(LXSN); mutant GFP cDNA was inserted just downstream of this promoter. Transfect SK-OV3 and MCF-7 cell lines with the new vector, P(LHGSN).

Results:

Three days after transfection with P(LHGSN), green fluorescence was observed in SK-OV3 cells while not in MCF-7 cells.

Conclusion:

HER2 promoter can control tumor specific expression of the reporter gene in ovarian cancer cell lines. With proper modification, the vector is expected to be useful in tumor specific gene therapy of ovarian cancers.

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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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