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Updated: Aug 6, 2026

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Published on: July 31, 2011
The use of recombinant antibodies in proteomics
L J Holt1, C Enever, R M de Wildt
1MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering, Hills Road, CB2 2QH, Cambridge, UK.
Abstract:
Recombinant antibodies are becoming increasingly important in the field of proteomics. Recent advances include the development of large phage-antibody libraries that contain high-affinity binders to almost any target protein, and new methods for high-throughput selection of antibody-antigen interactions. Coupled with a range of new screening technologies that use high-density antibody arrays to identify differentially expressed proteins, these antibody libraries can be applied to whole proteome analysis.
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