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High-Producers of Polygalacturonase Selected From Mutants Resistant to Rifampin in Alkalophilic Bacillus sp. NTT33
1Department of Microbiology and Immunology, School of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China
Enzyme and Microbial Technology
|October 12, 2000
Abstract:
Synthesis of polygalacturonases in alkalophilic Bacillus sp. which is sensitive to rifampin, was clearly repressed by glucose. Mutants resistant to glucose catabolic repression were selected. From spontaneous mutants, NTT33-cs52 and NTT33-cs301 produced polygalacturonase activities 57.2 to 82.4% higher than the wild type.