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Surface-immobilized dextran limits cell adhesion and spreading.

S P Massia1, J Stark, D S Letbetter

  • 1Department of Chemical, Bio, and Materials Engineering, Arizona State University, Tempe 85287-6006, USA. smassia@asu.edu

Biomaterials
|October 12, 2000
PubMed
Summary
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Dextran coatings offer a low-toxicity, effective alternative to polyethylene glycol (PEG) for biomaterials. These dextran surfaces resist cell adhesion, improving potential for advanced medical implants.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Polyethylene glycol (PEG) is commonly used for biomaterial coatings, but dextran offers advantages due to its multivalent properties.
  • Dextran is being explored as a low protein-binding, cell-resistant coating alternative to PEG for biomaterials.
  • Effective immobilization methods for dextran on biomaterial surfaces are needed, prioritizing simplicity and low toxicity.

Purpose of the Study:

  • To describe a simple, low-toxicity method for covalently immobilizing dextran onto material surfaces.
  • To evaluate the effectiveness of dextran coatings in preventing cell adhesion and spreading.
  • To compare cell adhesion on dextran-coated surfaces versus untreated and aminated surfaces.

Main Methods:

  • Covalent immobilization of 70 kDa dextran onto glass and polyethylene terephthalate (PET) surfaces using mild aqueous conditions and low-toxicity reagents.

Related Experiment Videos

  • Assessment of 3T3 fibroblast cell adhesion and spreading on untreated, aminated, and dextran-coated glass and PET surfaces.
  • Evaluation of coating performance in the presence of serum-borne cell adhesion proteins.

    • Main Results:

    • Dextran coatings were successfully immobilized onto glass and PET surfaces.
    • Dextran coatings significantly limited 3T3 fibroblast cell adhesion and spreading on both glass and PET.
    • The anti-fouling effect of dextran coatings was observed even in the presence of serum proteins.

    Conclusions:

    • A simple, low-toxicity method for dextran immobilization on biomaterials was established.
    • Dextran-based surface coatings demonstrate effective cell resistance, similar to PEG.
    • Dextran coatings hold promise for developing advanced biomaterials with controlled cell interactions for improved implant performance.