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Related Experiment Videos

Allostery in very large molecular assemblies.

K E van Holde1, K I Miller, E van Olden

  • 1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-7305, USA. vanholdk@ucs.orst.edu

Biophysical Chemistry
|October 12, 2000
PubMed
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Large macromolecules with many binding sites lack cooperativity because their functional units are subunits. Studies on molluscan hemocyanins reveal higher-order structure enables allosteric behavior in these subunits.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Small allosteric systems, like hemoglobin, exhibit cooperativity.
  • Large macromolecules with numerous binding sites (n) typically do not show cooperativity (nH), even approaching the theoretical limit.
  • This lack of cooperativity is attributed to cooperative units being substructures within the larger molecule.

Purpose of the Study:

  • To investigate the apparent anomaly of isolated substructures lacking cooperativity.
  • To explore the role of higher-order structure in enabling allosteric behavior in large macromolecules.
  • To study molluscan hemocyanins as a model system.

Main Methods:

  • Analysis of allosteric systems and cooperativity.
  • Investigation of macromolecular substructures.

Related Experiment Videos

  • Studies on molluscan hemocyanins.

    • Main Results:

    • Large macromolecules do not exhibit cooperativity due to their substructures acting as the cooperative unit.
    • Isolated substructures often do not display cooperativity on their own.
    • Molluscan hemocyanins demonstrate that higher-order molecular structure is essential for substructure allosteric function.

    Conclusions:

    • The higher-order structure of macromolecules provides the necessary framework for their substructures to exhibit allosteric behavior.
    • Cooperativity in large systems is dependent on the integrated structure, not just individual subunits.
    • Molluscan hemocyanins serve as a key example illustrating this principle.