Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcription through nucleosomes.

G Felsenfeld1, D Clark, V Studitsky

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.

Biophysical Chemistry
|October 12, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Gluino-Mediated Bottom Squark Production in pp[over ] Collisions at sqrt[s]=1.96 TeV.

Physical review letters·2009
Same author

Search for exclusive Z-boson production and observation of high-mass pp[over ]-->pgammagammap[over ]-->pl;{+}l;{-}p[over ] events in pp[over ] collisions at sqrt[s]=1.96 TeV.

Physical review letters·2009
Same author

First measurement of the tt[over ] differential cross section dsigma/dM_{tt[over ]} in pp[over ] collisions at sqrt[s]=1.96 TeV.

Physical review letters·2009
Same author

Patients' satisfaction with the nurse-led aural care clinic.

Journal of Ayub Medical College, Abbottabad : JAMC·2009
Same author

Apolipoprotein-E gene variants associated with cardiovascular risk factors in antipsychotic recipients.

European psychiatry : the journal of the Association of European Psychiatrists·2009
Same author

Search for top-quark production via flavor-changing neutral currents in W+1 jet events at CDF.

Physical review letters·2009
Same journal

Machine learning-driven identification of pan-PI3K inhibitors: A hybrid virtual screening approach combining naïve Bayesian classification, pharmacophore modeling, and consensus scoring-based molecular docking.

Biophysical chemistry·2026
Same journal

Optimizing grid preparation methods for TEM imaging of amyloid-forming proteins.

Biophysical chemistry·2026
Same journal

Biogenic reduction mechanisms in iron oxide nanoparticle synthesis: Strategies to mitigate microbial resistance.

Biophysical chemistry·2026
Same journal

Novel Pennisetum Alopecuroides-derived activated carbon for high-efficiency Tartrazine Removal: Box-Behnken optimization and DFT-assisted mechanistic insights.

Biophysical chemistry·2026
Same journal

Reactive molecular dynamics investigation of the first steps of coronavirus (COVID-19) viral-protein ligands fragment (SARS-CoV-2).

Biophysical chemistry·2026
Same journal

Probing the interactions between bovine hemoglobin and three berberine saturated fatty acid salts by multi-spectral techniques, conductimetry and molecular docking.

Biophysical chemistry·2026
See all related articles

Eukaryotic genes remain packaged in chromatin during transcription. RNA polymerase transcribes through nucleosomes by displacing, not releasing, the histone octamer, as confirmed by cryo-electron microscopy.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic genes are packaged into chromatin, a complex of DNA and proteins (histones).
  • Active genes retain chromatin structure, with histones chemically modified to facilitate transcription.
  • The mechanism by which RNA polymerase navigates nucleosomes during transcription remains a key question.

Purpose of the Study:

  • To investigate how RNA polymerase transcribes through nucleosomes.
  • To elucidate the fate of the histone octamer during transcription.

Main Methods:

  • Utilized model systems with single positioned nucleosomes on DNA templates.
  • Employed phage RNA polymerase (SP6) and yeast RNA polymerase III for transcription studies.
  • Applied electron cryo-microscopy for high-resolution structural analysis.

Related Experiment Videos

Main Results:

  • SP6 RNA polymerase does not release the histone octamer during transcription.
  • The histone octamer is displaced from its original binding site but remains associated with the DNA.
  • A detailed model for nucleosome traversal by RNA polymerase was proposed and refined.

Conclusions:

  • RNA polymerase can transcribe through nucleosomes by transiently displacing the histone octamer.
  • This mechanism is conserved across different RNA polymerases, including yeast RNA polymerase III.
  • Electron cryo-microscopy provides high-resolution insights into the transcription-nucleosome interaction.