Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Angiogenic therapy for the chronically ischemic lower limb in a rabbit model.

R Baffour1, J L Garb, J Kaufman

  • 1Baystate Medical Center, Springfield, Massachusetts 01199, USA.

The Journal of Surgical Research
|October 12, 2000
PubMed
Summary

Basic fibroblast growth factor (bFGF) enhances neovascularization and circulation at moderate doses, with effects lasting weeks. However, high bFGF doses may inhibit these beneficial processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using individual lung ultrasonography at the group level to define treatment thresholds in large groups of calves for tailoring antimicrobial use: A simulation approach.

Preventive veterinary medicine·2026
Same author

Optimising immunisations for children with needle phobia and/or developmental disorders: a retrospective audit to inform policy and practice in Australia.

Vaccine·2026
Same author

Lung ultrasonography used as a diagnostic test for respiratory disease diagnosis in calves: systematic review and meta-analysis using a Bayesian latent-class modelling approach.

Journal of dairy science·2026
Same author

A tailored COVID-19 vaccination pathway for children 5-11 years in Victoria, Australia.

Vaccine·2023
Same author

Drivers of COVID-19 vaccine hesitancy among women of childbearing age in Victoria, Australia: A descriptive qualitative study.

Vaccine: X·2022
Same author

Development and validation of a clinical respiratory disease scoring system for guiding treatment decisions in veal calves using a Bayesian framework.

Journal of dairy science·2022

Area of Science:

  • Regenerative Medicine
  • Vascular Biology
  • Pharmacology

Background:

  • Ischemic conditions impair limb circulation, necessitating therapeutic strategies for neovascularization.
  • Basic fibroblast growth factor (bFGF) is a potential agent for promoting new blood vessel formation.

Purpose of the Study:

  • To evaluate the long-term effectiveness of basic fibroblast growth factor (bFGF) in neovascularization after arterial ligation.
  • To determine the optimal dosage of bFGF for promoting angiogenesis and collateral circulation.

Main Methods:

  • Progressive femoral artery occlusion was induced in rabbits using an Ameroid constrictor.
  • Rabbits received intravenous injections of vehicle or varying doses of bFGF (3, 10, or 16 microg/kg/day) for 3 days.
  • Neovascularization was assessed using transcutaneous oximetry (TCPO(2)), angiography, and capillary density measurements.

Related Experiment Videos

Main Results:

  • Moderate bFGF doses (3 and 10 microg/kg/day) significantly increased TCPO(2) and capillary density compared to vehicle and high-dose groups.
  • Angiography revealed a higher number of vessels in moderate bFGF groups, indicating enhanced collateral formation.
  • High-dose bFGF (16 microg/kg/day) resulted in lower TCPO(2) values and potentially inhibited neovascularization.

Conclusions:

  • bFGF treatment demonstrated no adverse hemodynamic effects.
  • Moderate doses of bFGF effectively promote angiogenesis and improve circulation, with sustained effects.
  • High doses of bFGF may have an inhibitory effect on angiogenesis and collateral circulation development.