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The new serine-threonine kinase, Qik, is a target of the Qin oncogene

Y Xia1, Z Zhang, U Kruse

  • 1Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC239, La Jolla, California 92037, USA.

Biochemical and Biophysical Research Communications
|October 12, 2000
PubMed

Insights

Qin, an avian oncogenic protein, upregulates a serine-threonine kinase called Qin-induced kinase (Qik). Qik is a member of the AMPK/SNF1 kinase family and may be a shared target with Src.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • The winged helix transcription factor Qin is an avian homolog of mammalian brain factor 1 (BF-1).
  • Qin exhibits oncogenic potential.
  • Understanding Qin's downstream targets is crucial for cancer research.

Purpose of the Study:

  • To identify genes differentially expressed in chicken embryo fibroblasts (CEF) transformed by Qin.
  • To characterize a novel Qin-induced gene, Qik.
  • To explore potential shared targets between Qin and Src.

Main Methods:

  • Representational difference analysis (RDA) to identify differentially expressed genes.
  • In vitro kinase assays to assess Qik activity.
  • Analysis of Qik transcript levels in Src-transformed cells.

Main Results:

  • Representational difference analysis identified Qik (Qin-induced kinase) as a Qin-upregulated gene.
  • Qik is a serine-threonine kinase belonging to the AMPK/SNF1 kinase family.
  • Qik is ubiquitously expressed, rapidly upregulated upon Qin activation, and capable of autophosphorylation.
  • Elevated Qik transcripts were also found in Src-transformed cells.

Conclusions:

  • Qin induces the expression of the serine-threonine kinase Qik.
  • Qik is a functional kinase with autophosphorylation activity.
  • Qin and Src may share common downstream targets, including Qik.

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