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Decreased locomotor activity in mice carrying transgenic Fyn tyrosine kinase

H Ishibashi1, N Kojima, K Obata

  • 1Department of Physiological Sciences, Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585, Japan. ishibashi.cnb@tmd.ac.jp

Biochemical and Biophysical Research Communications
|October 12, 2000
PubMed

Insights

Mice with active Fyn tyrosine kinase have low movement. NMDA receptor antagonist MK-801 increased activity, suggesting Fyn phosphorylation of NMDA receptors controls locomotion.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Fyn tyrosine kinase is implicated in neuronal function.
  • NMDA receptors are crucial for synaptic plasticity and neuronal excitability.
  • The role of Fyn in regulating NMDA receptor function and behavior is not fully understood.

Purpose of the Study:

  • To investigate the role of Fyn tyrosine kinase in the regulation of spontaneous locomotor activity.
  • To determine if Fyn-mediated tyrosine phosphorylation of NMDA receptors influences locomotor behavior.

Main Methods:

  • Utilized transgenic mice with constitutively active Fyn tyrosine kinase.
  • Administered the NMDA receptor antagonist MK-801 to assess behavioral changes.
  • Measured spontaneous locomotor activity in mice under different conditions.

Main Results:

  • Mice expressing active Fyn tyrosine kinase exhibited significantly reduced spontaneous locomotor activity.
  • Treatment with MK-801 led to a notable increase in locomotor activity in these mice.
  • These results suggest a modulatory role for Fyn in NMDA receptor-dependent behaviors.

Conclusions:

  • Fyn tyrosine kinase activity is involved in suppressing spontaneous locomotor activity.
  • Tyrosine phosphorylation of NMDA receptors by Fyn plays a role in controlling locomotor behavior.
  • Targeting Fyn or NMDA receptor phosphorylation may offer therapeutic strategies for movement disorders.

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