Related Experiment Video
Updated: Aug 9, 2026

09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B
Abstract:
We describe a method for the direct selection of E. coli mutants restricting efficiency of suppression and misreading levels using a T4-coded nonsense suppressor. One mutant isolated has the phenotype expected for a restrictive mutant and may be ribosomal. Other possibilities are discussed.

