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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B

D Elseviers, L Gorini

    Molecular & General Genetics : MGG
    |January 1, 1975
    PubMed

    Abstract:

    We describe a method for the direct selection of E. coli mutants restricting efficiency of suppression and misreading levels using a T4-coded nonsense suppressor. One mutant isolated has the phenotype expected for a restrictive mutant and may be ribosomal. Other possibilities are discussed.

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