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Related Experiment Videos

Ca(2+)-activated Cl(-) current in sheep lymphatic smooth muscle.

H M Toland1, K D McCloskey, K D Thornbury

  • 1Smooth Muscle Group, Department of Physiology, Queen's University, Belfast BT9 7BL, United Kingdom.

American Journal of Physiology. Cell Physiology
|October 13, 2000
PubMed
Summary

A calcium-activated chloride current (I(Cl(Ca))) is crucial for spontaneous lymphatic smooth muscle activity. This chloride current influences electrical activity and fluid output in sheep lymphatics.

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Area of Science:

  • Physiology
  • Ion Channels
  • Smooth Muscle Biology

Background:

  • Sheep mesenteric lymphatic smooth muscle cells exhibit complex electrical activity.
  • Understanding the ion channels involved is key to comprehending lymphatic function.

Purpose of the Study:

  • To investigate the role of chloride currents in sheep mesenteric lymphatic smooth muscle.
  • To characterize the properties of a slowly developing current and its contribution to electrical activity.

Main Methods:

  • Perforated patch-clamp technique on sheep mesenteric lymphatic smooth muscle cells.
  • Voltage-clamp and current-clamp recordings.
  • Pharmacological manipulation with nifedipine, BAY K 8644, and anthracene-9-carboxylic acid (9-AC).

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Main Results:

  • A slowly developing current, distinct from L-type Ca(2+) current (I(Ca(L))), was identified.
  • This slow current was sensitive to chloride concentrations and blocked by 9-AC.
  • 9-AC also inhibited spontaneous transient depolarizations, action potentials, and reduced lymphatic fluid output.

Conclusions:

  • A calcium-activated chloride current (I(Cl(Ca))) plays a significant role in the electrical activity of sheep mesenteric lymphatics.
  • This chloride current is essential for spontaneous contractions and fluid pumping in lymphatic vessels.