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Related Experiment Videos

Elevation of exhaled ethane concentration in asthma.

P Paredi1, S A Kharitonov, P J Barnes

  • 1Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College School of Science, Technology and Medicine, London, United Kingdom.

American Journal of Respiratory and Critical Care Medicine
|October 13, 2000
PubMed
Summary

Exhaled ethane, a marker of oxidative stress, is elevated in asthma patients and reduced with steroid treatment. This finding suggests ethane may serve as a useful noninvasive biomarker for asthma management.

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Area of Science:

  • Biochemistry
  • Pulmonology
  • Biomarkers

Background:

  • Oxidative stress is implicated in asthma pathogenesis.
  • Ethane, a product of lipid peroxidation, is detectable in exhaled air.
  • Exhaled nitric oxide (NO) is a noninvasive marker of oxidative stress and inflammation in asthma.

Purpose of the Study:

  • To measure exhaled ethane levels in asthmatic subjects.
  • To compare exhaled ethane with exhaled nitric oxide (NO).
  • To investigate the correlation of exhaled ethane with asthma severity and steroid treatment.

Main Methods:

  • Exhaled ethane was collected via controlled exhalation and analyzed by gas chromatography.
  • Exhaled nitric oxide (NO) was measured using chemiluminescence.

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  • Asthmatic subjects (n=26) and control subjects (n=14) were studied.

    • Main Results:

    • Exhaled ethane levels were significantly higher in untreated asthmatic patients compared to steroid-treated patients and controls.
    • Exhaled ethane correlated positively with exhaled NO and air trapping (RV/TLC) in untreated asthmatics.
    • Untreated asthmatics with severe obstruction (FEV(1) < 60%) had higher ethane concentrations.

    Conclusions:

    • Exhaled ethane is elevated in asthma and decreases with steroid treatment.
    • Exhaled ethane may serve as a valuable noninvasive biomarker for oxidative stress in asthma.
    • The findings support the role of oxidative stress in asthma and highlight potential new diagnostic tools.