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Related Concept Videos

The Nucleus01:32

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
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Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
The Nucleus01:25

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
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Atomic Nuclei: Nuclear Spin01:08

Atomic Nuclei: Nuclear Spin

All atomic particles possess an intrinsic angular momentum, or 'spin'. Electrons, protons, and neutrons each have a spin value of ½, although protons and neutrons in nuclei may have higher half-integer spins owing to energetic factors.
Atomic nuclei have a net nuclear spin, , which can have an integer or half-integer value. In atomic nuclei, the spins of protons are paired against each other but not with neutrons, and vice versa. Consequently, an even number of protons does not contribute to...
The Nucleus01:25

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...

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Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

A myosin I isoform in the nucleus.

L Pestic-Dragovich1, L Stojiljkovic, A A Philimonenko

  • 1Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.

Science (New York, N.Y.)
|October 13, 2000
PubMed
Summary

A novel nuclear myosin I beta isoform was discovered, localizing with RNA polymerase II. This myosin isoform appears to interact with and potentially influence the transcription process.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Myosin I beta is typically known for its roles in cell shape and motility.
  • The presence and function of myosin isoforms in the nucleus are less understood.
  • Identifying nuclear proteins involved in gene regulation is crucial for understanding cellular processes.

Purpose of the Study:

  • To identify and characterize a nuclear-specific isoform of myosin I beta.
  • To investigate the functional interaction of nuclear myosin I beta with other nuclear components.
  • To determine the potential role of nuclear myosin I beta in gene transcription.

Main Methods:

  • Affinity purification and antibody generation against a unique N-terminal peptide of myosin I beta.
  • Confocal and electron microscopy to determine subcellular localization.
  • Co-immunoprecipitation assays to identify interacting proteins.
  • In vitro transcription assays to assess functional impact.

Main Results:

  • A nuclear isoform of myosin I beta with a unique 16-amino acid N-terminal extension was identified.
  • Nuclear myosin I beta was found to colocalize with RNA polymerase II.
  • This colocalization was sensitive to transcription inhibitors (alpha-amanitin and actinomycin D).
  • The antibody against nuclear myosin I beta co-immunoprecipitated RNA polymerase II and inhibited in vitro RNA synthesis.

Conclusions:

  • A distinct nuclear myosin I beta isoform exists and interacts with RNA polymerase II.
  • Nuclear myosin I beta may play a direct role in regulating transcription.
  • This finding opens new avenues for research into myosin's function in nuclear processes.