Physical mapping of the central terminator for transcription on the bacteriophage M13 genome

With the aid of in vitro transcription and translation studies it has been demonstrated that termination of transcription on bacteriophage M13 replicative form DNA occurs at a unique site which is located immediately distal to the 3'-end of gene VIII, the gene which codes for the major capsid protein. The position of this site has been mapped accurately on the enzyme cleavage maps by transcription of restriction fragments of M13 RF DNA. The central termination site was found to be located in restriction fragment Hap-B2 at 450 nucleotides from the 5'-end of its viral strand (0.77 fractional length clockwise from the unique Hind II enzyme cleavage site).