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Related Experiment Videos

Mutation in csrR global regulator reduces Streptococcus pyogenes internalization.

J Jadoun1, S Sela

  • 1Department of Human Microbiology, Tel Aviv University, Tel Aviv, 69978, Israel.

Microbial Pathogenesis
|October 14, 2000
PubMed
Summary

Group A Streptococcus internalization into epithelial cells is affected by the csr locus. This gene regulates hyaluronic acid capsule synthesis, influencing bacterial entry through known and unknown mechanisms.

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Group A Streptococcus (GAS) internalization into host epithelial cells is a key step in infection.
  • The role of specific GAS genes in this process remains incompletely understood.
  • Hyaluronic acid (HA) capsule expression is known to potentially impede bacterial entry.

Purpose of the Study:

  • To identify GAS genes involved in epithelial cell internalization using transposon mutagenesis.
  • To elucidate the mechanism by which the csr locus affects bacterial internalization.
  • To investigate the interplay between csr regulation and hyaluronic acid capsule synthesis in GAS entry.

Main Methods:

  • Transposon (Tn 916) mutagenesis was used to generate random mutations in GAS.

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  • A mutant with reduced internalization efficiency was selected for characterization.
  • Isogenic mutants deficient in csrR and/or hasA (hyaluronic acid capsule synthesis) were constructed and analyzed.
  • Bacterial adherence and internalization into HEp-2 cells were quantified.

    • Main Results:

    • A single Tn 916 insertion in the csr locus significantly reduced GAS internalization.
    • Mutations in csr derepressed hyaluronic acid capsule synthesis.
    • A hasA mutant exhibited enhanced adherence and internalization compared to wild-type and csrR mutants.
    • A csrR/hasA double mutant showed significantly reduced internalization compared to the hasA mutant.

    Conclusions:

    • The csr locus plays a significant role in GAS internalization into epithelial cells.
    • csrR influences streptococcal entry by modulating hyaluronic acid capsule expression.
    • An additional, yet uncharacterized, mechanism independent of capsule synthesis is also mediated by csrR affecting bacterial entry.