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Identification of ubiquitin as an immunophilin
1Health Sciences Department, Salisbury State University, Salisbury, Maryland, USA.
Biochemical and Biophysical Research Communications
|October 18, 2000
Abstract:
We have identified an 8.4 kDa minor immunophilin from calf thymus and Jurkat T cells as ubiquitin. It binds tacrolimus and sirolimus with K(d)'s of 0.8 and 0.08 nM, respectively. The binding of this protein to cyclosporin A is negligible. Binding of tacrolimus to two commercial sources of ubiquitin, a bovine product, and a recombinant human ubiquitin, was also demonstrated after HPLC purification of the purchased preparations.