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Related Experiment Videos

Piglets born after vitrification of embryos using the open pulled straw method.

F Berthelot1, F Martinat-Botté, A Locatelli

  • 1INRA Equipe Ovocytes et Développement, PRMD, Nouzilly, 37380, France. berthelo@tours.inra.fr

Cryobiology
|October 18, 2000
PubMed
Summary

The ultrarapid open pulled straw (OPS) vitrification method effectively cryopreserved unhatched porcine blastocysts. This technique shows promise for preserving embryos from both Large White hyperprolific and Meishan pig breeds.

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Area of Science:

  • Animal Science
  • Reproductive Biology
  • Cryobiology

Background:

  • Porcine embryo cryopreservation is crucial for genetic resource conservation and efficient breeding programs.
  • The open pulled straw (OPS) vitrification method offers a rapid approach to cryopreservation.

Purpose of the Study:

  • To evaluate the efficacy of an ultrarapid OPS vitrification method for cryopreserving porcine embryos (morulae and blastocysts) from Large White hyperprolific (LWh) and Meishan (MS) breeds.
  • To compare the viability of vitrified/warmed embryos using two different cryoprotectant dilution media: TCM (TCM199 + NBCS) and PBS (PBS + NBCS).

Main Methods:

  • Porcine morulae and unhatched blastocysts from LWh and MS gilts were vitrified using the OPS method with either TCM or PBS as the dilution medium.
  • Equilibration involved ethylene glycol, dimethyl sulfoxide, and sucrose, followed by warming with a four-step sucrose dilution.

Related Experiment Videos

  • Embryo viability was assessed by in vitro development to hatched blastocysts and by in vivo birth rates after embryo transfer.

    • Main Results:

    • In PBS, MS blastocysts showed significantly higher in vitro viability (67%) compared to LWh blastocysts (27%).
    • In TCM, no significant difference was observed between MS (43%) and LWh (41%) blastocysts, but viability was lower than controls.
    • Vitrification of morulae using PBS resulted in low viability for both breeds (11% LWh, 14% MS).
    • In vivo transfer of vitrified/warmed MS blastocysts resulted in a 55% farrowing rate, with a median of 4 piglets for MS and 5 for LWh recipients.

    Conclusions:

    • The ultrarapid OPS vitrification method is suitable for cryopreserving unhatched porcine blastocysts.
    • The choice of dilution medium (TCM vs. PBS) significantly impacts blastocyst viability, with PBS favoring Meishan embryos.
    • The OPS method demonstrates potential for successful cryopreservation and subsequent development of porcine embryos.