Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regular, rodlike, macromolecular structures in Artemia salina.

P Nieuwenhuysen, F Rijsheuvels, H Slegers

    Biochimica Et Biophysica Acta
    |May 24, 1979
    PubMed
    Summary

    Researchers discovered rod-like macromolecular structures in brine shrimp (Artemia salina). These particles, likely nucleoprotein complexes, were visualized using electron microscopy and purified via sucrose gradient centrifugation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An SEM compatible plasma cell for in situ studies of hydrogen-material interaction.

    The Review of scientific instruments·2020
    Same author

    Phase I study of emactuzumab single agent or in combination with paclitaxel in patients with advanced/metastatic solid tumors reveals depletion of immunosuppressive M2-like macrophages.

    Annals of oncology : official journal of the European Society for Medical Oncology·2019
    Same author

    SIESTA: A high current ion source for erosion and retention studies.

    The Review of scientific instruments·2018
    Same author

    Thoracic aortic aneurysm complicated by secondary aortoesophageal fistula after thoracic endovascular aortic repair : a case report.

    Acta gastro-enterologica Belgica·2018
    Same author

    Contact sites between the inner and outer mitochondrial membranes in stunned versus hibernating myocardium.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Ventilation - how often are we correct?

    Anaesthesia and intensive care·2012

    Area of Science:

    • Marine Biology
    • Biochemistry
    • Molecular Biology

    Background:

    • Artemia salina, commonly known as brine shrimp, are widely used model organisms in biological research.
    • Previous studies have indicated the presence of various macromolecular components within Artemia salina.
    • Understanding the composition of these organisms is crucial for various applications, including aquaculture and toxicological studies.

    Purpose of the Study:

    • To identify and characterize novel macromolecular structures within Artemia salina.
    • To determine the physical and chemical properties of these structures.
    • To elucidate the potential biological role of these particles.

    Main Methods:

    • Electron microscopy with negative staining was employed to visualize the structures.

    Related Experiment Videos

  • Purification of the particles was achieved through sucrose gradient centrifugation in the presence of EDTA.
  • Size distribution analysis was performed on electron micrographs.

    • Main Results:

    • Regular, rodlike, macromolecular structures were consistently observed in Artemia salina from diverse origins and developmental stages.
    • These particles were successfully isolated from the postmitochondrial supernatant.
    • Electron microscopy revealed distinct morphology, and size distribution measurements provided quantitative data on the particles.

    Conclusions:

    • The identified rodlike structures are likely nucleoprotein complexes.
    • These findings contribute to the understanding of molecular organization within Artemia salina.
    • Further research is warranted to fully characterize the function and composition of these nucleoprotein complexes.