Related Experiment Videos
Selecting optimal antisense reagents.
1Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK. msohail@bioch.ox.ac.uk
Advanced Drug Delivery Reviews
|October 18, 2000
Summary
Selecting effective antisense experiment target sites is challenging due to RNA secondary structures. This review highlights
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Antisense experiments require precise target site selection for efficacy.
- RNA secondary structures impede base pairing, complicating target identification.
- Conventional methods like sequence-walking and computational design have limited success.
Purpose of the Study:
- To review and summarize empirical methods for selecting antisense target sites.
- To highlight significant advancements in target site selection strategies.
- To provide an overview of current approaches for optimizing antisense experiments.
Main Methods:
- Review of empirical target site selection methods.
- Summary of 'global' methods for transcript mapping.
- Discussion of endoribonuclease H (RNase H) and oligonucleotide scanning arrays.
Main Results:
- Empirical methods offer alternatives to conventional approaches.
- 'Global' methods utilizing RNase H and scanning arrays show significant promise.
- Effective target site selection is critical for successful antisense outcomes.
Conclusions:
- Advancements in target site selection are crucial for antisense technology.
- RNase H-based and oligonucleotide scanning array methods represent significant progress.
- Improved target site selection enhances the reliability and success of antisense experiments.