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Related Experiment Videos

Selecting optimal antisense reagents.

M Sohail1, E M Southern

  • 1Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK. msohail@bioch.ox.ac.uk

Advanced Drug Delivery Reviews
|October 18, 2000
PubMed
Summary
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Selecting effective antisense experiment target sites is challenging due to RNA secondary structures. This review highlights

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Antisense experiments require precise target site selection for efficacy.
  • RNA secondary structures impede base pairing, complicating target identification.
  • Conventional methods like sequence-walking and computational design have limited success.

Purpose of the Study:

  • To review and summarize empirical methods for selecting antisense target sites.
  • To highlight significant advancements in target site selection strategies.
  • To provide an overview of current approaches for optimizing antisense experiments.

Main Methods:

  • Review of empirical target site selection methods.
  • Summary of 'global' methods for transcript mapping.

Related Experiment Videos

  • Discussion of endoribonuclease H (RNase H) and oligonucleotide scanning arrays.

    • Main Results:

    • Empirical methods offer alternatives to conventional approaches.
    • 'Global' methods utilizing RNase H and scanning arrays show significant promise.
    • Effective target site selection is critical for successful antisense outcomes.

    Conclusions:

    • Advancements in target site selection are crucial for antisense technology.
    • RNase H-based and oligonucleotide scanning array methods represent significant progress.
    • Improved target site selection enhances the reliability and success of antisense experiments.