Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SBE-TAGS: an array-based method for efficient single-nucleotide polymorphism genotyping.

J N Hirschhorn1, P Sklar, K Lindblad-Toh

  • 1Whitehead Institute/MIT Center for Genome Research, One Kendall Square, Building 300, Cambridge, MA 02139, USA.

Proceedings of the National Academy of Sciences of the United States of America
|October 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insights from the largest diverse ancestry sex-specific disease map for genetically predicted height.

NPJ genomic medicine·2025
Same author

Proceedings of Fox River Valley Association.

Chicago medical examiner·2023
Same author

Allele frequency spectrum of known ankylosing spondylitis associated variants in a Swedish population.

Scandinavian journal of rheumatology·2021
Same author

Assessment of differentially methylated loci in individuals with end-stage kidney disease attributed to diabetic kidney disease: an exploratory study.

Clinical epigenetics·2021
Same author

Roan, ticked and clear coat patterns in the canine are associated with three haplotypes near usherin on CFA38.

Animal genetics·2021
Same author

Transcriptomes from German shepherd dogs reveal differences in immune activity between atopic dermatitis affected and control skin.

Immunogenetics·2020
Same journal

Costunolide ameliorates autoimmune uveitis by targeting USP15 to suppress TNF-α-induced retinal endothelial inflammation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A ligandable PNT domain establishes ERG as a directly targetable oncogenic driver in prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in <i>Clostridioides difficile</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

The structure of correlated variability reflects task-relevant information in sensory neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Shared neurogenetic substrates of nonplanning impulsivity and procrastination.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

HIV-1 capsid interactions with Nuclear Pore Complex components support nuclear entry via affinity gradient.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

A new method called single base extension-tag array on glass slides (SBE-TAGS) enables parallel genotyping of numerous single nucleotide polymorphisms (SNPs). This technique overcomes limitations in current genotyping methods, facilitating large-scale genetic studies.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Generating human single-nucleotide polymorphisms (SNPs) is no longer a bottleneck for genetic disease studies.
  • Current genotyping methods are limited to studying only a few SNPs at a time.
  • Progress in genetic studies is hindered by the inability to genotype large numbers of SNPs efficiently.

Purpose of the Study:

  • To introduce a novel method for parallel genotyping of SNPs.
  • To address the limitations of existing low-throughput genotyping techniques.
  • To enable high-throughput SNP genotyping for large-scale genetic research.

Main Methods:

  • Developed a method named single base extension-tag array on glass slides (SBE-TAGS).
  • Utilized single base extension (SBE) with bifunctional primers containing locus-specific sequences and unique tags.

Related Experiment Videos

  • Employed a generic tag array on glass slides for "demultiplexing" genotyped products.

    • Main Results:

    • Achieved highly multiplexed SNP genotyping.
    • Demonstrated the simplicity and cost-effectiveness of the SBE-TAGS method.
    • Obtained approximately 99% accuracy in genotyping over 100 SNPs, resulting in over 5,000 genotypes.

    Conclusions:

    • SBE-TAGS offers a simple, inexpensive, and accurate solution for parallel SNP genotyping.
    • The method significantly advances the capacity for large-scale genetic studies.
    • Facilitates efficient and high-throughput analysis of genetic variations.