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A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
Optimization of conditions for safflower cell culture and accumulation of cellicolous product tocopherols
1Bio-environment Engineering Institute of Zhejiang University, Huajiachi, Hangzhou, China.
Abstract:
The culture of calli, which were induced from cotyledon of Carthamus tinctorius L, was able to synthesize tocopherols. Results determined by NP-HPLC showed that the main component was alpha-tocopherol. When 0.1% casein was added into MS medium, and 2,4-D concentration was adjucted to 0.30 mg/L and 6-BA to 1.80 mg/L, alpha-, beta- and gamma-forms of tocopherol reached 4.1706, 0.3120 and 0.1650 microg/g fresh calli, respectively. The effective tocopherol content was 4.4091 microg/g fresh calli, and was 57 times higher than that in the control.
