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Published on: May 4, 2016
In vitro genotoxicity testing of ARASCO and DHASCO oils
L M Arterburn1, K D Boswell, T Lawlor
1Martek Biosciences Corporation, 6480 Dobbin Road, Columbia, MD 21043, USA.
Summary
ARASCO and DHASCO oils, rich in specific fatty acids, were evaluated for mutagenicity. These oils demonstrated no genotoxic potential in multiple in vitro assays, indicating safety.
Area of Science:
- Biotechnology
- Toxicology
- Nutritional Science
Background:
- ARASCO and DHASCO oils are microbial triglycerides.
- These oils are rich in arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3).
Purpose of the Study:
- To assess the mutagenic and genotoxic potential of ARASCO and DHASCO oils.
- Evaluate safety for potential applications.
Main Methods:
- In vitro mutagenesis assays including Ames test, mouse lymphoma assay, and chromosomal aberration assay.
- Assays were performed with and without metabolic activation.
Main Results:
- ARASCO and DHASCO oils showed no mutagenic activity in the Ames reverse mutation assay.
- No mutagenicity was observed in the mouse lymphoma forward mutation assay.
- The oils exhibited no clastogenic activity in chromosomal aberration assays.
Conclusions:
- ARASCO and DHASCO oils do not appear to possess genotoxic potential.
- Findings suggest these oils are safe from a genotoxicity perspective.
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